Skip to main content
Fig. 4 | BMC Bioinformatics

Fig. 4

From: A voting mechanism-based linear epitope prediction system for the host-specific Iridoviridae family

Fig. 4

Antigenicity test by ELISA assays. 10 μg of different synthetic peptides were coated on a 96-well microplate, respectively. For antigenicity and cross antigenicity tests, all synthetic peptides were hybridized by rabbit anti-rTGIVmcp or rabbit anti-rGIVmcp antibodies and detected by goat anti-rabbit IgG (h + l) conjugated horseradish peroxidase (HRP) antibody. The figures of a, b, and c represent the responses of synthetic peptides for VIV subfamily including Lymphocystivirus157–166, Megalocystivirus157–166, and Ranavirus152–161 (exclusive LEs for VIV group), respectively; the figures of d, e, f, and g represent the responses of synthetic peptides for IIV subfamily including Iridovirus193–203, Chloriridovirus92–102, Iridovirus226–236, and Chloriridovirus125–135 (exclusive LEs for IIV group), respectively. **-immunization (GIVmcp antibody): before immunization by rGIVmcp (pre-immune serum). +immunization (GIVmcp antibody): after immunization by rGIVmcp (immunized serum). -immunization (TGIVmcp antibody): before immunization by rTGIVmcp (pre-immune serum). +immunization (TGIVmcp antibody): after immunization by rTGIVmcp (immunized serum). ABS 630 nm: Absorbance (read at 630 nm)

Back to article page