Fig. 2

Schematic illustration of “AND” gate A2. The schematic diagram of cleaving reaction mediated by DNAzyme and the principle of signal recovery apparatus are shown in (a). For “AND” gate A2, strand K-4 and M-4 are inputs hybridizing to form DNAzme H implementing catalytic cleavage with Mg²⁺, cleaving NAin-f on the brown site. G0 was formed and F-s was released after cleavage, resulting in fluorescent intensity increased as shown in (c). There is also the true value table of “AND” logic gate A2 depicted in (d). b The PAGE analysis of logic operations based on DNAzyme cleavage (DNA concentration was 2 μM, volume 10 μL). The error bars represent the standard deviations of three independent experiments