TY - JOUR AU - Wang, Zhe AU - Hu, Junming AU - Johnson, W. Evan AU - Campbell, Joshua D. PY - 2019 DA - 2019/05/02 TI - scruff: an R/Bioconductor package for preprocessing single-cell RNA-sequencing data JO - BMC Bioinformatics SP - 222 VL - 20 IS - 1 AB - Single-cell RNA sequencing (scRNA-seq) enables the high-throughput quantification of transcriptional profiles in single cells. In contrast to bulk RNA-seq, additional preprocessing steps such as cell barcode identification or unique molecular identifier (UMI) deconvolution are necessary for preprocessing of data from single cell protocols. R packages that can easily preprocess data and rapidly visualize quality metrics and read alignments for individual cells across multiple samples or runs are still lacking. SN - 1471-2105 UR - https://doi.org/10.1186/s12859-019-2797-2 DO - 10.1186/s12859-019-2797-2 ID - Wang2019 ER -