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Fig. 3 | BMC Bioinformatics

Fig. 3

From: Software tool for internal standard based normalization of lipids, and effect of data-processing strategies on resulting values

Fig. 3

Linear regression comparing the log10 of normalized lipid levels calculated using different workflows and ions. A slope of 1 and R2 close to 1 are expected if the methods or ions both result in similar normalized lipid levels. The panels show normalized levels calculated using smoothed versus non-smoothed peak heights (smoothing was done as the final step in MZmine; n = 184; a), peak area versus peak height (n = 184; b), positive versus negative polarity using peak area (n = 51; c), and sodium adducts versus the major adduct observed in positive polarity using peak area (n = 76; d). For d, sodium adducts were compared to protonated adducts except in the case of neutral lipids which formed ammoniated adducts

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BMC Bioinformatics

ISSN: 1471-2105

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