Software tool for internal standard based normalization of lipids, and effect of data-processing strategies on resulting values

Table 2 Comparison of different lipid quantification software which can be applied to UHPLC-HRMS/MS data

	Output	IS: Class Specific^a	Multiple IS per Class^b	Response Factors^c	Vendor Specific	License	Modular^d
Lipid Data Analyzer	Concentration^e	Yes	Yes	No	No	Open Source	No
MZmine 2	Normalized Peak Intensities	No	_	No	No	Open Source	No
LipidMatch Normalizer	Concentration^e	Yes	Yes	No	No	Open Source	Yes
SimLipid	Concentration^e	Yes	Yes	No	No	Purchase	No
LipidSearch	Concentration^e	Yes	No	No	No	Purchase	No

^aCan internal standard be matched to features for quantification based on lipid class?
^bCan multiple internal standards for a single lipid class be used?
^cAre response factors based on lipid structures and resulting ionization efficiencies employed?
^dCan the tool be used with various feature finding and identification software?
^eNote that for these software while outputs are technically in units of concentration, they should not be interpreted as quantitative, but rather as normalized abundances to class representative internal standards (relative quantification)

ISSN: 1471-2105