Fig. 1

Overview of the aneuvis workflow for analyzing numerical chromosomal variation. a An experiment begins with the quantification of number of chromosomes per cell using either FISH, SKY, or sc-WGS. b Next, the number of chromosomes per cell within each treatment group is stored as a cell x chromosome matrix, where the entries indicate the number of inferred copies of a chromosome in a cell. c Aneuvis incorporates information from the experimental design as well as from chromosomal copy number matrices to determine whether differences exist between treatment groups. A table of descriptive statistics summarized by group and by chromosome is automatically generated and available for download. Visual representations of the relationship in aneuploidy between different groups are also automatically generated. A permutation-based approach allows the user to conclude whether there is a statistically significant difference in the ploidy characteristics between treatment groups