Fig. 2
Heat map of the 7-dimensional Pareto front from MOFA analysis of anaerobic mixotrophic metabolism of 3 aromatic compounds in R. palustris. a 2333 unique Pareto-optimal solutions identified during our analysis. Each biological objective was examined at intervals equaling 1/5 maximum normalized value. b Select Pareto-optimal solutions from the MOFA analysis of growth, carbon fixation/carbon efficiency and H2 production in RP when growing on a variety of different aromatic compounds. The predicted growth archetypes (A, B & C) have greater growth rates than measured values (D to I). At the measured growth rates, our MOFA analyses predict presence of many metabolic phenotypes. Some (D, F & H) use lower amounts of carbon and fix greater amounts of CO2. These phenotypes do not produce H2 gas. Others (E, G & I), import extra carbon and use the energy from metabolizing this resource to produce small amounts of H2 gas. As with aliphatic metabolism, maximum production of H2 results in cessation of growth and full oxidation of the carbon source (J, K & L)