Fig. 1
From: Variable cellular decision-making behavior in a constant synthetic network topology
Synthetic implementation of the CAA circuit. a The CAA topology consists of two auto-regulating, mutually-inhibiting transcription factors (TA, TB). b Schematic for the TetR monomer which comprises dimerization, drug-binding, and DNA-binding domains. c Mutations in the DNA-binding and dimerization domains enable the simultaneous, non-interfering use of an altered-specificity TetR (Set 1) along with the original TetR (Set 0). d Dimerization is possible only between monomers of the same set. e Circuit implementing the CAA topology. The CAA model comprises two opposing sides, TA, TB; here, TA is represented by the transcriptional activator tTA0, the repressor tTS0, and the promoter tetO0. Similarly, TB is represented by the transcriptional activator tTA1, the repressor tTS0, and the promoter tetO1. The activators are fused to either GFP or mCherry to allow for tracking via fluorescence measurements. The two sides are delivered on separate plasmids that integrate into yeast chromosomes