Fig. 2
From: Variable cellular decision-making behavior in a constant synthetic network topology
CAA circuit can generate a spectrum of behaviors and requires strong repression for discrete decisions. Comparison of behaviors observed under three different genetic perturbations. Only clones with the tetO-2x strong repression circuit are able to yield discrete decisions, with cells expressing high levels of either GFP or mCherry, but not both. Images and flow cytometry data captured at the 16-h time-point. Flow cytometry data from individual clones of each circuit variant were merged to create cumulative response profiles. a Weak repression for 2x tet operator sites. b Strong repression for 2x tet operator sites. c Strong repression for 7x tet operator sites