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Table 2 Assembly results on SARS-CoV aligned and recruited metagenomic data

From: TAR-VIR: a pipeline for TARgeted VIRal strain reconstruction from metagenomic data

Aligned

Tool

# Contigs

N50

Genomes covered (%)

Mismatch rate (%)

Bowtie2 L,0,-0.9

TAR-VIR

58

505

19.7

0.02

 

SGA

56

505

20.1

0.03

 

SPAdes

34

569

12.9

0.16

 

SAVAGE

54

455

17.5

0.0

Recruited

Tool

# Contigs

N50

Genomes covered (%)

Mismatch rate (%)

 

TAR-VIR

7

29,676

98.9

0.0

 

SGA

13

26,729

98.9

0.0

 

SPAdes

14

15,882

92.1

0.51

Bowtie2 L,0,-0.9

SAVAGE

22

12,445

97.0

0.0

Aligned

Tool

# Contigs

N50

Genomes covered (%)

Mismatch rate (%)

 

TAR-VIR

84

1192

55.1

0.0

 

SGA

85

1027

56.5

0.0

 

SPAdes

67

1012

44.6

0.12

BWA B:1

SAVAGE

68

669

32.3

0.0

Recruited

Tool

# Contigs

N50

Genomes covered (%)

Mismatch rate (%)

BWA B:1

TAR-VIR

6

29,706

99.5

0.0

 

SGA

18

12,638

99.5

0.0

 

SPAdes

21

10,353

89.2

0.39

 

SAVAGE

56

5140

89.3

0.0

  1. The default assembly tool in TAR-VIR is PEHaplo. Definition of the metrics can be found in “Data properties and evaluation metrics” section