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Table 5 Assembly evaluation results on aligned and recruited reads using the genomes of HBV, HCV, and HPgV as references

From: TAR-VIR: a pipeline for TARgeted VIRal strain reconstruction from metagenomic data

Align

Tool

Bowtie2 aligned

Bowtie2 Recruited

  

Contig #

N50

Genome cov. (%)

Contig #

N50

Genome cov. (%)

5%

TAR-VIR

11

920

27.3

97

3643

82.3

 

SGA

14

645

26.8

63

675

68.4

 

SPAdes

5

1177

27.6

15

3636

79.6

 

SAVAGE

13

698

21.6

49

806

40.4

10%

TAR-VIR

61

794

67.4

31

2635

84.0

 

SGA

26

663

56.4

72

706

69.5

 

SPAdes

15

1251

65.4

19

3373

79.3

 

SAVAGE

30

631

40.6

32

915

26.5

15%

TAR-VIR

97

939

80.9

14

3579

83.1

 

SGA

56

617

57.7

74

722

70.2

 

SPAdes

20

1689

77.6

16

3986

81.0

 

SAVAGE

32

639

29.9

24

999

27.6

20%

TAR-VIR

38

1852

84.5

77

5678

86.4

 

SGA

78

661

59.5

374

537

64.5

 

SPAdes

23

2,710

83.8

10

4830

84.6

 

SAVAGE

19

671

19.1

15

823

5.5

  1. ‘cov.’ is the abbreviation for ‘coverage’. The default assembly component in TAR-VIR is PEHaplo