Fig. 2

System diagram of PaSS. The sequencing profile (or sequencing error model) can be generated from real sequencing data and  its alignment to a reference genome. Reads can be simulated based on the reference genome and the sequencing profile (or error model). For each read, a fragment of sequence from the reference genome is selected first then the sequencing errors will be added according to the profile, which includes preset error rates for two ends of the read, the number of passes, read length distribution, context based error model and error size