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Fig. 4 | BMC Bioinformatics

Fig. 4

From: COMBImage2: a parallel computational framework for higher-order drug combination analysis that includes automated plate design, matched filter based object counting and temporal data mining

Fig. 4

COMBO-MF Flowchart. THRESHOLD TUNING:(1)-(3) Matched filtering on training images; (4)-(5) Cross validation for optimal detection threshold. INTRA-PLATE ANALYSIS:(1) Image datastore selected by the user; (2) COMBO-Pick specification imported by the user; (3) MapReduce-based intra-plate quality control; (4)-(5) MapReduce-based quantification of apoptotic-like cells; (6) Table (CSV) with results; (7) Temporal graphics (EPS, PDF). INTER-PLATE ANALYSIS:(1) Intra-plate analysis employed separately for all replicates; (2) Results from (1) gathered and parsed; (3) Outlier removal based on the Inter-Plate QC as performed by COMBO-C; (4) Table (CSV) with merged inter-plate replicate values; (5) Temporal graphics (EPS, PDF)

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BMC Bioinformatics

ISSN: 1471-2105

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