COMBImage2: a parallel computational framework for higher-order drug combination analysis that includes automated plate design, matched filter based object counting and temporal data mining

Table 1 Modular comparison of COMBImage2 and COMBImage

Readout	Module	Description	Layout	Analysis	COMBImage2	COMBImage
	COMBO-Pick	automated experimental design	P, PE, E		+	-
TLVM	COMBO-MF	matched filter based object counting	P, PE, E	intra,inter	+	-
	COMBO-C	changes in cell confluence	P	intra	+	+
			P	inter	+	-
			PE, E	intra,inter	+	-
	COMBO-M	changes in cell morphology	P	intra	+	+
			P	inter	+	-
			PE, E	intra,inter	+	-
CVA	COMBO-V	cell viability & synergy analyses	P	intra	+	+
			P	inter	+	-
			PE, E	intra,inter	+	-
TLVM, CVA	COMBO-Mine	temporal data mining	PE, E	intra, inter	+	-

Abbreviations are defined as follows. TLVM: time-lapse video microscopy, CVA: cell viability assay, P: pairwise (only pairs of drugs are evaluated in a checkerboard format), PE: partially exhaustive (particular subsets among a panel of drugs are evaluated), E: exhaustive (all possible subsets among a panel of drugs are evaluated), intra: intra-plate analysis (experiment performed in a single experimental plate), inter: inter-plate analysis (experiment replicated in several plates)

ISSN: 1471-2105