Fig. 1From: WellInverter: a web application for the analysis of fluorescent reporter gene dataRelation between the measurements of fluorescence and absorbance in reporter gene experiments and the activity of the host gene and the concentration of the protein it encodes. a: Co-expression of the host and reporter gene, together encoding a single fusion protein. b: Idem, but the host gene and reporter gene code for two distinct proteins. c: Co-regulation of the host and reporter genes, which share the same transcriptional and translational regulatory sequences (promoter and ribosome binding site, RBS). d: Idem, but only transcriptional regulation is shared between the host and reporter genes as indicated by the different colors of the RBSs. e-f: As in c-d, but the reporter gene is carried on a plasmid rather than in the chromosome. In order to avoid visual clutter, the transcription and translation processes in the figure have been collapsed into a single protein synthesis stepBack to article page