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Fig. 1 | BMC Bioinformatics

Fig. 1

From: WellInverter: a web application for the analysis of fluorescent reporter gene data

Fig. 1

Relation between the measurements of fluorescence and absorbance in reporter gene experiments and the activity of the host gene and the concentration of the protein it encodes. a: Co-expression of the host and reporter gene, together encoding a single fusion protein. b: Idem, but the host gene and reporter gene code for two distinct proteins. c: Co-regulation of the host and reporter genes, which share the same transcriptional and translational regulatory sequences (promoter and ribosome binding site, RBS). d: Idem, but only transcriptional regulation is shared between the host and reporter genes as indicated by the different colors of the RBSs. e-f: As in c-d, but the reporter gene is carried on a plasmid rather than in the chromosome. In order to avoid visual clutter, the transcription and translation processes in the figure have been collapsed into a single protein synthesis step

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