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Fig. 10 | BMC Bioinformatics

Fig. 10

From: WellInverter: a web application for the analysis of fluorescent reporter gene data

Fig. 10

Relation between growth rate and reporter concentration for the pRM promoter. The steady-state growth rate and reporter concentration have been computed for each condition considered (Additional file 2), by taking the mean and standard deviation of the estimates of these quantities over the interval where the cultures are in steady-state exponential growth. The scatter plot shows the mean ± the standard deviation for each condition (blue points and lines, respectively). The growth rates are expressed in doublings per h and the reporter concentrations have been normalized with respect to the estimate for M9 minimal medium with 0.1% glucose. The red dots connected by the red curve are the predicted growth rates and reporter concentrations for a constitutive promoter [28]. The predictions and the data are in very good correspondence, as exemplified by the high R2 value (0.93). The R2 value was computed as one minus the ratio of the residual sum of squares divided by the total sum of squares [51]. In order to account for data points below 0.6 doublings/h and above 2.5 doublings/h, we linearly extrapolated the model predictions

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