Fig. 8

Computation of reporter concentrations. Mean absorbance and reporter concentration curves and an uncertainty band defined by the standard deviation for a group of wells containing WT pRM-gfp cultures growing in M9 minimal medium with 0.1% glucose (blue) or 0.1% maltose (green). Upper: Reporter concentration estimates obtained by dividing fluorescence by absorbance. Lower: Smoothed estimates of reporter concentrations obtained by computing the protein concentration while setting the degradation constant of the host protein equal to the degradation constant of the GFP reporter in the Parameters window (0.00065 min ⁻¹). Notice that the two estimates are not shown on the same scale