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Fig. 17 | BMC Bioinformatics

Fig. 17

From: AutoCryoPicker: an unsupervised learning approach for fully automated single particle picking in Cryo-EM images

Fig. 17

Particle picking using EMAN2 and AutoCryoPicker. a The manually selected reference particles of the Apoferritin dataset that were used for automated particle picking with EMAN2. b Zoomed-in view of the reference particles for the Apoferritin dataset. c EMAN2 automatic picking result based on threshold value = 0.0 using the first tested image of the Apoferritin dataset. d EMAN2 automatic picking result based on threshold value = 0.5 using the first tested image of the Apoferritin dataset. e EMAN2 automatic picking result based the threshold value = 2.3 using the first tested image of the Apoferritin dataset. Red dots mark missed particles). f Ground truth of first tested image of the Apoferritin dataset. Yellow dots mark valid particles. g EMAN2 automatic picking result based the threshold value = 2.3 using the second tested image of the Apoferritin dataset. Red dots mark missed particles). h Ground truth of second tested image of the Apoferritin dataset. Yellow dots mark valid particles. i The manually selected reference particles of the KLH dataset that were used for automated picking of top-view (circular) particles with EMAN2. j EMAN2 automatic picking result based the threshold value = 0.5 using the first tested image of the KLH dataset. Red squares mark the false positives and the yellow dots the missing particles. k Zoomed-in view of the automatically picked particles (threshold value = 0.5) for first tested image of the KLH dataset. l EMAN2 automatic picking result based the threshold value = 0.5 using the second tested image of the KLH dataset. Red squares mark the false positives, and the yellow dots mark the missing particles (top-view). m Particle picking result from AutoCryoPicker using the first tested image of the Apoferritin dataset. Red ‘+’ mark the center of each particle and blue circles the top-view detected particles in the cryo-EM image. n Particle picking result from AutoCryoPicker using the second tested image of the Apoferritin dataset. Red ‘+’ mark the center of each particle and blue circles the top-view detected particles in the cryo-EM image. o Particle picking result from AutoCryoPicker using the first tested image of the KLH dataset. Red ‘+’ marks the center of each particle, blue circles the top-view detected particles in the cryo-EM image, and the yellow squares the side-view detected particles in the cryo-EM image. p Particle picking result from AutoCryoPicker using the second tested image from the KLH dataset. Red ‘+’ marks the center of each particle, blue circles the top-view detected particles in the cryo-EM image, and the yellow squares the side-view detected particles in the cryo-EM image

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