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Table 2 Overview of the constraints for the undesired space (T) used in formulations involving enumeration of constrained minimal cut sets

From: Comparison of pathway analysis and constraint-based methods for cell factory design

Formulation Product constraint Substrate constraint Coupling constraint Biomass constraint
MCSe \( \frac{v_p}{v_s}\le {y}_{min} \) vs ≤ smax vatp ≥ m None
MCSf vs = smax None
MCSw vp ≤ ε vs = smax None vb ≥ F. bmax