From: Comparison of pathway analysis and constraint-based methods for cell factory design
Formulation | Product constraint | Substrate constraint | Coupling constraint | Biomass constraint |
---|---|---|---|---|
MCSe | \( \frac{v_p}{v_s}\le {y}_{min} \) | v_{s} ≤ s_{max} | v_{atp} ≥ m | None |
MCSf | v_{s} = s_{max} | None | ||
MCSw | v_{p} ≤ ε | v_{s} = s_{max} | None | v_{b} ≥ F. b_{max} |