Table 3 Phenotype prediction methods used to obtain the performance metrics featured in this work
From: Comparison of pathway analysis and constraint-based methods for cell factory design
Method | Objective function | Constraints | Productivity metrics |
|---|---|---|---|
pFBA | Min (|v|) subject to Max (vbiomass) | Environmental conditions | Maximum cell growth = vbiomass |
Production rate = vproduct | |||
Biomass-product coupled yield: \( BPCY(v)=\frac{v_{biomass}\ast {v}_{product}}{v_{substrate}} \) | |||
FVA | Min (vproduct) | Environmental conditions vbiomass ≥ x Max (vbiomass) | Production robustness at x % growth = Min (vproduct) |