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Fig. 1 | BMC Bioinformatics

Fig. 1

From: Prediction of regulatory targets of alternative isoforms of the epidermal growth factor receptor in a glioblastoma cell line

Fig. 1

Western blot analysis of the two glioblastoma cell lines U251MG and SF767. U251MG is a PTEN mutant and PIK3CA wild-type cell line and SF767 is a PTEN wild-type and PIK3CA (E545K) mutant cell line. Cells were treated for 24 hours with different levels of the EGFR-ligand EGF (0-50 ng/ml). The levels of HER2 and EGFR are reduced by EGF-dependent degradation of the formed and internalized EGF-HER2/EGFR complexes. The activation of AKT-protein (phosphorylation of the Ser473) is detectable in an EGF-dependent manner in cell line SF767, whereas the pAKT level is constantly high in cell line U251MG. These observations indicate that the EGFR-signaling pathway is inducible in cell line SF767, but not in cell line U251MG. Anti- β-actin staining was done as a loading control, and BIRC5 (survivin) was used as an indicator for proliferation activity

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