Fig. 2

Schematic principle of gcOpt. a represents an exemplary production envelope of a wild-type strain showing no GC, with the black dashed and dotted lines denoting the lower production rate bounds of possible mutant strains. The red dashed line denotes the optimization principle of gcOpt, which is maximization of the minimally guaranteed production rate at a medium fixed growth rate. b is a production envelope of a reaction deletion mutant strain showing the best possible GC, where the grey area represents the production envelope of the wild-type strain which is inaccessible for the mutant strain