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Fig. 1 | BMC Bioinformatics

Fig. 1

From: SRAssembler: Selective Recursive local Assembly of homologous genomic regions

Fig. 1

SRAssembler workflow. The pipeline takes as input a query sequence and DNA sequencing read files, which are split into smaller files in a preprocessing step, allowing for parallelization of alignments. In the initial round, SRAssembler aligns the processed reads against the query (DNA or protein) sequence to identify reads that could potentially contribute to a homologous contig. Matched reads are then assembled into contigs, which are used as queries in the next round of searching the read libraries. The reads found in recursive rounds are reassembled, extending the edges of the contigs until no further matching reads are found or until user criteria for success are met (usually a finite number of rounds or complete coverage of the query). In the final round a spliced alignment program aligns the original query to the final contigs, predicting contigs that are good matches for the query and their homologous gene structure(s)

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