Fig. 5
From: iMAP: an integrated bioinformatics and visualization pipeline for microbiome data analysis
Features of the assembled and aligned sequences. Merging the forward and reverse reads resulted in sequences with 250 nucleotides (a). The 250-nucleotide sequence length is perfectly in-line with the targeted V4 region of the 16S rRNA gene. Most of the overlap fragments were 150 nucleotides long (b) and had zero mismatches (c). The query length (d) and alignment length (e) showed a high percent identity at 90 and 100% (f)