Fig. 4

Assessing normalization of RNA-seq data using scatterplot matrices. Illustrating normalization checks with data from [22]. The collective deviation of genes from the x=y line instantly reveals that the RNA-seq dataset was not thoroughly normalized using within-lane normalization (subplot a). However, within-lane normalization followed by between-lane normalization sufficiently normalized the data (subplot b). The authors who developed these normalization methods showed that the later approach generated a lower false-positive DEG call rate in this dataset