Fig. 2
Quality-based sorting. a The schematic representation of the adult zebrafish spinal cord indicates the four vertebral levels (V4, V12, V20, and V28) from where tissue sections were obtained. An exemplary tissue section is shown, depicting the locality of gray (inside profile) and white (outside profile) matter. The fluorescence image shows myelin-rich regions labeled with GFP (green, white matter) and mitochondria-rich regions labeled with dsRed (magenta, gray matter). For each probed region (red rectangles), four to five force-distance (FD) curves were recorded. Additional FD curves for white matter were recorded outside of these regions. In total, ten specimens at the age of six months were measured (see [18] for details). b The first violin plot shows the distribution of apparent Young’s moduli for gray and white matter. The second and third plot show the same data filtered with the Extra Trees regressor at a classification threshold of 4.5. c The first violin plot is identical to that in (B). The second plot shows the 150 top-rated (Extra Trees regressor) data points for gray and white matter. The third plot shows the remainder of the data points. The number of FD curves in each violin plot is indicated with N1 for gray matter and N2 for white matter