Fig. 3
From: A multiscale mathematical model of cell dynamics during neurogenesis in the mouse cerebral cortex
Experimental cell counts: protocol and results a Dorsal view of a wild-type E16.5 mouse brain. The level of the coronal sections used in this study is indicated by a white double arrow. b Coronal cryosection of an E13.5 mouse brain corresponding to the level of section shown in a. The window used for the quantification is framed. w: width; th: thickness. c-e Confocal scanning micrographs (1 μm single optical section) of coronal sections of the dorsal telencephalon at E13.5 (c&d) and E16.5 (e). c Immunostaining with antibodies against Pax6 and Tbr2. DAPI counterstaining is used to label all the nuclei. White arrows show a selection of Pax 6+Tbr2+ nuclei (IPPs). d Immunostaining with antibodies against Tbr2, PH3 (to label cells in M-phase), Ctip2 (to label neurons). e Immunostaining with antibodies against Ctip2 (to label deep layer neurons) and Satb2 (to label upper layer neurons). IZ: Intermediate Zone. Scale bars: 1 mm in a, b, 50 μm in c, d, 150 μm in e. f-h Experimental cell counts for control (WT, blue dots) and Ftm−/− (KO, red dots) samples