Fig. 1

Schematic representation of EUCs and SASDs and their ability to resolve the ambiguity of cross-linker assignation. Here, a simplified example based on a homo-dimer is shown where cross-linking results in three cross-links (1–2, 1–3, and 2–3). During sampling, multiple homo-dimer models can be generated with different relative orientation between the two subunits (only three models are shown). The ability to correctly classify cross-links as inter- or intra-subunit, combined with an appropriate scoring function, helps in generating the correct model of the homo-oligomer