Fig. 6
From: Cytokit: a single-cell analysis toolkit for high dimensional fluorescent microscopy imaging
T cell CD3 (blue), CD4 (red), and CD8 (green) intensity. a First row of 5 images in 5 × 5 experiment grid. b Single tile image with corresponding cell and nucleus segmentation, where cells are defined as a fixed radius away from the nucleus in the absence of a plasma membrane stain. c Center zoom on (b) showing co-expression of CD3 and CD4 (magenta) and CD3 and CD8 (cyan) as well as debris in DAPI channel