Fig. 4

Enrichment analysis and TF ratio. Gene ontology (GO) and KEGG enrichment analyses were performed for cold (A) and heat (B) stress. There were 12 and 7 clusters for cold and heat stress, respectively. More GO and KEGG terms were enriched in six early phase clusters for cold stress and three early phase clusters for heat stress than in six later phase clusters for cold stress and four later phase clusters for heat stress. Functional terms related to transcription factors were enriched in early phase clusters. The terms are GO biological process term “transcription, DNA-templated”, the GO molecular function term “DNA-binding transcription factor activity”, and the GO cellular process term “nucleus”, which are highlighted by red color. We also examined how the proportion of TFs in the cluster changes as the response progresses for cold (C) and heat (D) stress. The result showed that the fraction of TFs was high in the early phase, while the TF fraction was decreased with the passage of time