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Table 2 Summary of the different protocols utilized to generate extracts of differing condition. For each protocol, three levels were generated such that the extracts generally became more compromised as the level increased (i.e., due to increasing enzyme concentration, increasing incubation time, increasing sonication cycle number, etc.)

From: Statistical modeling of STR capillary electrophoresis signal

 

Number of whole blood donors (n) for each condition

 

level

Condition

I

II

III

N/A

Untreated

   

n=50

(mU)

6

12

24

 

rDNase I

n=35

n=35

n=35

 

(min)

15

30

45

 

Fragmentase®

n=15

n=15

n=15

 

(cycles)

2

10

30

 

Sonication

n=14

n=14

n=14

 

(min)

15

60

120

 

UV Damage

n=22

n=22

n=22

 

(μL)

15

22

35

 

Humic Acid

n=22

n=22

n=22

 
  1. The number of donors from which DNA extracts were obtained and subjected to the various protocols is indicated