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Table 2 Comparison of clustering results from manual clustering performed by 5 experts using FlowJo and automated clustering using flowEMMi

From: flowEMMi: an automated model-based clustering tool for microbial cytometric data

 

# clusters

Range of abundances (%)

Cell numbers (%)

# congruent clusters

   

Foreground

Background

 

flowEMMi

12

1.56 - 20.72

71.6

28.4

12

User 1

13

0.25 - 27.7

76.5

23.5

10

User 2

15

0.21 - 30.0

82.1

17.9

11

User 3

13

0.24 - 28.2

79.1

20.9

11

User 4

16

0.26 - 31.2

90.7

9.3

11

User 5

15

0.22 - 32.1

91.6

8.4

11

  1. Compared were i) the number of clusters that were found, ii) the range of the abundance values of all clusters, iii) the cell numbers of foreground/background cell and iv) the number of congruent clusters that were found by the user and flowEMMi, respectively. Congruent clusters are cell clusters having the same or similar mean values in both parameters (FSC and DAPI-Fluorescence)