SCOPIT: sample size calculations for single-cell sequencing experiments

BMC Bioinformatics

Table 2 Comparison of Independent Approximation and Exact Calculations.

Subpopulation frequency	# of subpopulations	Cells required (exact)	Cells required (approx.)
0.1	6	186	186
0.2	3	85	85
0.3	2	53	53
0.1	8	191	191
0.2	4	87	87
0.4	2	39	39
0.1	9	193	193
0.3	3	55	55
0.1	10	195	194
0.2	5	89	89
0.5	2	30	30

The number of cells required to achieve a 95% certainty of sampling sufficiently many cells from each subpopulation. The number of cells was calculated in two ways: by an exact calculation, and by an approximate calculation in which the counts of different subpopulations were assumed to be independent

ISSN: 1471-2105

Subpopulation frequency	# of subpopulations	Cells required (exact)	Cells required (approx.)
0.1	6	186	186
0.2	3	85	85
0.3	2	53	53
0.1	8	191	191
0.2	4	87	87
0.4	2	39	39
0.1	9	193	193
0.3	3	55	55
0.1	10	195	194
0.2	5	89	89
0.5	2	30	30

Subpopulation frequency	# of subpopulations	Cells required (exact)	Cells required (approx.)
0.1	6	186	186
0.2	3	85	85
0.3	2	53	53
0.1	8	191	191
0.2	4	87	87
0.4	2	39	39
0.1	9	193	193
0.3	3	55	55
0.1	10	195	194
0.2	5	89	89
0.5	2	30	30

Subpopulation frequency	# of subpopulations	Cells required (exact)	Cells required (approx.)
0.1	6	186	186
0.2	3	85	85
0.3	2	53	53
0.1	8	191	191
0.2	4	87	87
0.4	2	39	39
0.1	9	193	193
0.3	3	55	55
0.1	10	195	194
0.2	5	89	89
0.5	2	30	30