Fig. 3

The key. Using the default settings, the Manhattan++ plot generates a key for the plot. For keys #5–8, there are 2–400 variants per cell in the matrix (using different configuration file from Fig. 2). The software calculates the maximum count based on the overall matrix. For key #5, there are 6264 cells in the plot. Key #6 tells us that the cell contains 1 or more high impact variant(s) and there are 4 such cells in the plot. Key #7 gives us information on those cells where there are one or more variant(s) with MAF < 5% and there are 1226 such cells. Key #8 tells us that in 2–400 variants, there is one variant with MAF < 5% and is also high impact. If the cell contains 2 variants, one with MAF < 5% and one that is high impact, then the cell will also get key #8