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Table 1 The four different reference sequences used to guide the reconstruction of the western-grey kangaroo mitochondrial amplicon from short sequencing reads. For each circular mitochondrial genome, the genome coordinates of the extracted region are indicated as well as its length. The percentage identity to the western-grey amplicon is calculated on the homologous regions only, i.e. the non-aligned sections at the beginning and the end of the alignment are not taken into account

From: Effective machine-learning assembly for next-generation amplicon sequencing with very low coverage

Species

Genbank accession

Start position

End position

Length of extracted region (bp)

Percentage identity to western-grey amplicon

Eastern-grey kangaroo

NC_027424

11,749

47

5,186

91.4%

Swamp wallaby

KJ868164

11,000

1,200

7,075

86.8%

Tasmanian devil

JX475466

11,000

1,200

7,336

65.7%

House mouse

NC_005089

11,000

1,200

6,500

59.0%