Skip to main content
Fig. 1 | BMC Bioinformatics

Fig. 1

From: NASQAR: a web-based platform for high-throughput sequencing data analysis and visualization

Fig. 1

NASQAR platform architecture. A cluster of virtual machines at NYU Abu Dhabi serves NASQAR applications to multiple concurrent users. Applications are containerized and managed on the cluster using Docker and Swarm, while Traefik load-balances requests among available server nodes. Functionality includes merging gene counts, conversion of gene IDs to gene names, analysis of differential mRNA expression, metagenomics analysis, and functional enrichment analysis. Applications for bulk expression analysis include DESeq2, limma, and EdgeR. Single-cell RNAseq analysis with Seurat Wizards is built on top of the Seurat R package and includes options for filtering, normalization, dimensionality reduction (PCA), clustering, and UMAP/t-SNE. Enrichment analysis includes applications for Gene Set Enrichment Analysis (GSEA) and Over-representation Analysis (ORA) built using the clusterProfiler R package

Back to article page