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Table 1 Summary statistics of aligning PacBio/ONT reads to short-read assembly graphs (constructed by SPAdes with kmer size equal to 77)

From: SPAligner: alignment of long diverged molecular sequences to assembly graphs

Aligner

MR(%)

AI (%)

T (h:m)

M (Gb)

 

MR(%)

AI (%)

T (h:m)

M (Gb)

E. coli PacBio

 

E. coli ONT

vg

29

90

00:41

4.1

 

58

88

00:33

3.3

GraphAligner

85.3

86.7

00:01

0.1

 

82.2

86.1

00:01

0.2

GA try-all-seeds

86

86.6

00:25

0.3

 

83.6

86

01:43

4.8

SPAligner

88.3

86.5

00:04

0.3

 

86.6

86.1

00:03

0.3

S. cerevisiae PacBio

 

S. cerevisiae ONT

vg

15

90

28:03

91

 

70

47

42:59

85

GraphAligner

53.7

86.5

00:01

0.2

 

65.7

81.1

00:01

0.2

SPAligner

56.5

85.9

00:38

0.7

 

61.6

82.1

00:16

0.6

C. elegans PacBio

 

C. elegans ONT

vg

10

91

76:26

306

 

15

89

60:58

309

GraphAligner

84.1

86.8

00:03

1.8

 

63.6

86.6

00:03

1.8

SPAligner

84.6

86.5

01:06

1.2

 

66

86.4

00:34

1.3

  1. Every dataset consists of 10k reads longer than 2Kbp (except E. coli ONT dataset with 7k reads of appropriate length). All runs performed in 16 threads. Due to high fragmentation of assembly graph resulting from available C. elegans Bristol N2 Illumina sequencing data, we used simulated Illumina reads to obtain C. elegans assembly graph