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Table 2 Amino acid substitutions between Paragon and Chinese Spring coding sequences of TraesCS5A01G531700.1. AA = Amino acid; “first variety” refers to the first variety listed in the corresponding “Comparison” column for each row

From: AutoCloner: automatic homologue-specific primer design for full-gene cloning in polyploids

Comparison Position AA in first variety AA in second variety
Paragon & Chinese Spring 270 M T
Paragon & Chinese Spring 292 Q P
Paragon & Chinese Spring 552 I V
Paragon & Chinese Spring 731 P H
Paragon & Chinese Spring 760 A V
Paragon & Apogee 35 L F
Paragon & Apogee 64 F V
Paragon & Apogee 66 E K
Paragon & Apogee 69 E A
Paragon & Apogee 270 M T
Paragon & Apogee 292 Q P
Paragon & Apogee 552 I V
Paragon & Apogee 731 P H
Paragon & Apogee 760 A V
Chinese Spring & Apogee 35 L F
Chinese Spring & Apogee 64 F V
Chinese Spring & Apogee 66 E K
Chinese Spring & Apogee 69 E A