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Fig. 3 | BMC Bioinformatics

Fig. 3

From: A cell-level quality control workflow for high-throughput image analysis

Fig. 3

Performance of cell-level QC, assay β. a Examples of sampled images grouped by their phenotypes. Sampled images were grouped into seven phenotypes with five examples shown for each. For visualization purposes, only a representative region of the original well image is displayed. b A comparison of phenotype score before and after cell-level QC for different cell lines. From top to bottom are examples of raw images and their phenotype masks before and after QC. Red dash line box shows a well dominated by artifacts (ARcell > 70%) and removed before downstream analysis. c Examples of wells containing heterogeneous subpopulations to show minority phenotypes (e.g., cells labeled in magenta) were not mistakenly labeled as artifacts by our cell-level QC approach. In all masks, nuclei with phenotype of interest are labeled in magenta, otherwise blue; detected artifacts are marked in green

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