Fig. 3From: Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!Histogrammed E values from 4 × 104 simulations of the 9-point model described in text, fitted directly to Eq. 2 and converted to differences for fitting to Eq. 3. The boxes show the results from fitting to the normal distribution, in which the counts were weighted inversely as the count (Poisson approximation)Back to article page