Skip to main content
Fig. 3 | BMC Bioinformatics

Fig. 3

From: METROID: an automated method for robust quantification of subcellular fluorescence events at low SNR

Fig. 3

Signal extraction by different filtering methods. a Representative simulated AP trace recovered by different BSS methods. Signals are time-shifted for better visualization. Black continuous line is the original noise-free AP signal, gray continuous line is a noisy observation (SNR = − 10 dB), blue dashed line is ICA output, red dashed line is PCA output, green dot-dashed line is wICA output, orange dot-dashed line is wPCA output and purple dotted line is 5th order low-pass Butterworth filter. Matching style arrows indicate AP signal onset time. b Relative error for maximal AP amplitude (Er_Amp) as function of SNR for AP signal. Gray stars represent Er_Amp means calculated directly from noisy data (N = 120 samples for each mean), blue circles represent ICA means, red squares represent PCA means, green inverted triangles represent wICA means, orange triangles represent wPCA means and purple crosses represent 5th order low-pass Butterworth filter. Shaded regions correspond to standard errors of the mean. c Correlation coefficient (r) means for AP signal calculated in AP active interval (N = 12 samples for each mean). d Representative simulated step recovered by different methods. Signals are amplitude-shifted for better visualization. Line color and styles represent the same outputs as in (a). Matching style arrows indicate step signal baseline amplitude. e Er_Amp for step function

Back to article page