Table 2 Parameters

k_s.cdG=33.5/min

scaled synthesis rate of cdG

this study

k_d,cdG=100/min

scaled degradation rate of cdG

this study

K₁=0.5μM

dissociation constant for product inhibition

[40]

K_m1=1500 μM

binding affinity of GTP

this study

K_m2=0.06μM

binding affinity of cdG

[40]

[DgcB]=0.7 μM

scaled DgcB level

[41]

[basal PDEs]=0.2 μM

scaled basal PDE level

this study

k_s.(p)ppGpp=170μM/min

synthesis rate of (p)ppGpp

this study

k_d.(p)ppGpp=160μM/min

degradation rate of (p)ppGpp

this study

K₂=75μM

binding affinity of NPr ∼P

this study

K₃=10μM

dissociation constant of EIIA ∼P

this study

K_SpoT=4

constant of SpoT activity

this study

K_m3=1000μM

binding affinity of GTP

this study

K_m4=2000μM

binding affinity of (p)ppGpp

this study

K₄=75.63μM

parameters of glutamine inhibition

[42]

ε=0.1

k_s.GTP=1500/min

synthesis rate of GTP

this study

k_d.GTP=100/min

degradation rate of GTP

this study

[ EI]_T=10μM

total enzymes levels

[34–36]

[ NPr]_T=30μM

[ EIIA]_T=30μM

k₁=52.4/min

phosphotransfer constants

this study, [27, 42]

k₋₁=67.2/min

k_±2=1.2×10⁴/(min·μM)

[27, 33]

k_±3=3.7×10³/(min·μM)

\(K_{\mathrm {d1}}=\frac {k_{b_{-1}}}{k_{b_{1}}}=350\mu \mathrm {M}\)

dissociation constants

[27]

\(K_{\mathrm {d2}}=\frac {k_{b_{-2}}}{k_{b_{2}}}=670\mu \mathrm {M}\)