Skip to main content

Table 2 Hypothetical data used to generate Figure 4b. Calculation of predicted relative expression, \( \hat{R} \), values follows \( {10}^{m\left({x}_0-x\right)} \), where m =  − 0.139, and the values are plotted in Figure 4d. x0 = 9.85 is the mean x. The 95% confidence interval for the slope m is (− 0.212, − 0.066)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

α1 concentration (pg/mL) \( {\Delta C}_q^{(w)} \)   α1 concentration (pg/mL) \( \hat{R} \) Lower
Confidence Interval
Upper Confidence Interval
8.7 0.683   8.7 0.692 0.572 0.840
8.9 0.568   8.9 0.738 0.630 0.866
9.4 0.598   9.1 0.787 0.695 0.892
9.5 0.424   9.3 0.839 0.766 0.920
9.8 0.537   9.5 0.894 0.844 0.948
10.1 0.428   9.7 0.953 0.930 0.977
10.2 0.337   9.9 1.016 1.008 1.025
10.4 0.491   10.1 1.083 1.039 1.129
10.6 0.360   10.3 1.155 1.071 1.244
10.9 0.334   10.5 1.231 1.104 1.371
    10.7 1.313 1.138 1.511
    10.9 1.399 1.173 1.666