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Table 2 Hypothetical data used to generate Figure 4b. Calculation of predicted relative expression, \( \hat{R} \), values follows \( {10}^{m\left({x}_0-x\right)} \), where m =  − 0.139, and the values are plotted in Figure 4d. x0 = 9.85 is the mean x. The 95% confidence interval for the slope m is (− 0.212, − 0.066)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

α1 concentration (pg/mL)

\( {\Delta C}_q^{(w)} \)

 

α1 concentration (pg/mL)

\( \hat{R} \)

Lower

Confidence Interval

Upper Confidence Interval

8.7

0.683

 

8.7

0.692

0.572

0.840

8.9

0.568

 

8.9

0.738

0.630

0.866

9.4

0.598

 

9.1

0.787

0.695

0.892

9.5

0.424

 

9.3

0.839

0.766

0.920

9.8

0.537

 

9.5

0.894

0.844

0.948

10.1

0.428

 

9.7

0.953

0.930

0.977

10.2

0.337

 

9.9

1.016

1.008

1.025

10.4

0.491

 

10.1

1.083

1.039

1.129

10.6

0.360

 

10.3

1.155

1.071

1.244

10.9

0.334

 

10.5

1.231

1.104

1.371

   

10.7

1.313

1.138

1.511

   

10.9

1.399

1.173

1.666