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Table 3 Calculation of predicted relative expression, \( \hat{R} \), values using hypothetical data from Table 1. Calculation of \( \hat{R} \) values follows \( {10}^{m\left(x-{x}_0\right)} \), where m =  − 0.139, and these values are plotted in Figure 4e. x0 = 10.9 is the largest x value. The 95% confidence interval for the slope m is (− 0.162, − 0.117)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

α1 concentration (pg/mL) \( \hat{R} \) Lower Confidence Interval Upper Confidence Interval
8.7 0.495 0.440 0.553
8.9 0.527 0.474 0.583
9.1 0.562 0.511 0.616
9.3 0.599 0.551 0.650
9.5 0.639 0.593 0.686
9.7 0.681 0.639 0.724
9.9 0.726 0.689 0.764
10.1 0.774 0.742 0.806
10.3 0.825 0.799 0.851
10.5 0.880 0.861 0.898
10.7 0.938 0.928 0.948
10.9 1 1 1