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Table 3 Calculation of predicted relative expression, \( \hat{R} \), values using hypothetical data from Table 1. Calculation of \( \hat{R} \) values follows \( {10}^{m\left(x-{x}_0\right)} \), where m =  − 0.139, and these values are plotted in Figure 4e. x0 = 10.9 is the largest x value. The 95% confidence interval for the slope m is (− 0.162, − 0.117)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

α1 concentration (pg/mL)

\( \hat{R} \)

Lower Confidence Interval

Upper Confidence Interval

8.7

0.495

0.440

0.553

8.9

0.527

0.474

0.583

9.1

0.562

0.511

0.616

9.3

0.599

0.551

0.650

9.5

0.639

0.593

0.686

9.7

0.681

0.639

0.724

9.9

0.726

0.689

0.764

10.1

0.774

0.742

0.806

10.3

0.825

0.799

0.851

10.5

0.880

0.861

0.898

10.7

0.938

0.928

0.948

10.9

1

1

1