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Table 5 Hypothetical data used to generate Figure 6A. Calculation of predicted relative expression, \( \hat{R_B} \) , values follows \( {R}_A^m \), where m = 0.367, and these values are plotted in Figure 6B. x0 = 0.457 is the mean x. The 95% confidence interval for the slope m is (0.278, 0.456)

From: Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

\( {\Delta C}_{q;A}^{(w)} \) \( {\Delta C}_{q;B}^{(w)} \) \( {\Delta \Delta C}_{q;A}^{(w)} \) RA \( \hat{R_B} \) Lower Confidence Intervals Upper Confidence Intervals
0.633 0.882 0.177 0.666 0.861 0.831 0.893
0.568 0.845 0.112 0.774 0.910 0.889 0.931
0.534 0.833 0.078 0.837 0.937 0.922 0.952
0.474 0.829 0.018 0.961 0.985 0.982 0.989
0.487 0.811 0.031 0.932 0.975 0.968 0.981
0.428 0.806 −0.029 1.068 1.024 1.018 1.030
0.387 0.798 − 0.070 1.174 1.061 1.045 1.076
0.398 0.766 −0.059 1.144 1.051 1.038 1.063
0.360 0.771 −0.097 1.249 1.085 1.064 1.107
0.296 0.755 −0.161 1.447 1.145 1.108 1.184