Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

Table 5 Hypothetical data used to generate Figure 6A. Calculation of predicted relative expression, \( \hat{R_B} \) , values follows \( {R}_A^m \), where m = 0.367, and these values are plotted in Figure 6B. x₀ = 0.457 is the mean x. The 95% confidence interval for the slope m is (0.278, 0.456)

\( {\Delta C}_{q;A}^{(w)} \)	\( {\Delta C}_{q;B}^{(w)} \)	\( {\Delta \Delta C}_{q;A}^{(w)} \)	R_A	\( \hat{R_B} \)	Lower Confidence Intervals	Upper Confidence Intervals
0.633	0.882	0.177	0.666	0.861	0.831	0.893
0.568	0.845	0.112	0.774	0.910	0.889	0.931
0.534	0.833	0.078	0.837	0.937	0.922	0.952
0.474	0.829	0.018	0.961	0.985	0.982	0.989
0.487	0.811	0.031	0.932	0.975	0.968	0.981
0.428	0.806	−0.029	1.068	1.024	1.018	1.030
0.387	0.798	− 0.070	1.174	1.061	1.045	1.076
0.398	0.766	−0.059	1.144	1.051	1.038	1.063
0.360	0.771	−0.097	1.249	1.085	1.064	1.107
0.296	0.755	−0.161	1.447	1.145	1.108	1.184

ISSN: 1471-2105