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Fig. 2 | BMC Bioinformatics

Fig. 2

From: IonCRAM: a reference-based compression tool for ion torrent sequence files

Fig. 2

Base calling based on flow signals. The upper part shows an example DNA fragment to be sequenced. The second part shows the sequence of nucleotides in the flow cycle. It also shows the values of the sensed flow signals and the called bases. A flow signal value exceeding a certain threshold means that a base had hybridized to the template and the corresponding base in the flow cycle is reported. If the flow signal value doubles, this indicates a polymer of identical bases. The base calling software calibrates the signal values and decides the length of the polymer

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