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Table 2 Validation of cBASE. 176 expression plasmids were compared with the corresponding amplified cDNA sequences. An expression recommendation by cBASE was considered false-negative if the software recommended against using the plasmid, although upon manual inspection the differences between plasmid and amplified were negligible (and most likely due to sequencing quality artifacts)

From: Brain antibody sequence evaluation (BASE): an easy-to-use software for complete data analysis in single cell immunoglobulin cloning

cBASE analysis results # of chains Comments
correct expression recommendation 145/176  
false-negative expression recommendation 13/176 mostly related to circumscibed low sequencing quality either in the plasmid or in the amplified cDNA Ig sequence
no automatic recommendation 18/176 mostly related to general low sequencing quality either in the plasmid or in the amplified cDNA Ig sequence
false-positive recommendation 0/176