Fig. 1
eRF1-1 shows Cys126-centered redox sensitivity in vitro. a Phylogenetic tree of Cys126 of A. thaliana eRF1-1 as example for ConCysFind output trees. The phylogenetic tree represents the grade of similarity between the most similar protein sequences, found in each of the 21 proteomes compared to the input sequence of eRF1-1. Thus it has a phylogenetic aspect and indicates functional significance. eRF1-1 Cys126 represents a newly identified fully conserved cysteine in the plant kingdom, indicating a potential redox-sensitive functionality in vivo for this particular residue. b Western Blot of eRF1-1 in redox gradient. eRF1-1 was subjected to distinct ratios of DTTox and DTTred, spanning from fully oxidising (≥ 250 mV) to fully reducing (≤ 410 mV) conditions. Besides the eRF1-1-His6 monomer (ca. 50 kDa), eRF1-1 oligomers were visualised with anti His6-antibody. c eRF1-1 wildtype protein and Cys-to-Ser variants C126S, C388S and C404S under fully oxidising (ox) and reducing (red) conditions after Western blotting and detection with anti His6-antibody. Significant differences in oligomerisation pattern under oxidising conditions are indicated with black arrows