Fig. 1
From: DeepCryoPicker: fully automated deep neural network for single protein particle picking in cryo-EM
Overview of the DeepCryoPicker procedure. a The general workflow of the training particle-selection based unsupervised scheme and single particle picking based on deep learning scheme. The gray part of the workflow shows the micrographs data collection. The blue part of the workflow shows the fully automated training particles-selection using clustering algorithms. The red part of the workflow shows the general flow of the single particle picking using a deep classification network. The yellow part of the workflow shows the external testing part of the DeepCryoPicker. b 3D Cryo-EM map of the Apoferritin. c Picked particle from an Apoferritin micrograph [27]. d 3D Cryo-EM map of KLH is viewed from the top. e Picked particle from a KLH micrograph [26] showing the top view (circular particle). f 3D Cryo-EM map of KLH is viewed from the side. g Picked particle from a KLH micrograph [26] showing the side-view (square particle). h 3D Cryo-EM map of the 80S ribosome. i Picked particle from a ribosome micrograph [28]. j 3D Cryo-EM map of β-galactosidase. k Picked particle from a β-galactosidase micrograph [29]