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Table 1 Table of the times and the RAM memory peaks reached during the RNA-Seq analysis for the eight pipelines

From: ARPIR: automatic RNA-Seq pipelines with interactive report

 

Alignment

Quantification

DEA

Time (h)

RAM (Gb)

1° pipeline

TopHat2

Cufflinks

cummeRbund

35

3.3

2° pipeline

Hisat2

StringTie

Ballgown

4

4.3

3° pipeline

Hisat2

featureCounts

DESeq2

4

4.3

4° pipeline

Hisat2

featureCounts

edgeR

4

4.3

5° pipeline

STAR

Cufflinks

cummeRbund

5

30

6° pipeline

STAR

featureCounts

DESeq2

4

30

7° pipeline

STAR

featureCounts

edgeR

4

30

8° pipeline

Kallisto

Kallisto

Sleuth

2

2.2

  1. The longest pipeline was found to be TopHat2-Cufflinks-cummeRbund, while the shorter kallisto-sleuth. The RAM memory peaks are maintained in all cases except for STAR pipelines below 5 Gb